mouse anti mrp2  (Novus Biologicals)

Journal: Regenerative Therapy

Article Title: Reprogramming canine cryopreserved hepatocytes to hepatic progenitor cells using small molecule compounds

doi: 10.1016/j.reth.2024.09.003

Figure Lengend Snippet: Immunofluorescent cell staining. Immunostaining demonstrated cHep cell differentiation into hepatic progenitor cells. On day 21, ALB and MRP2 were weakly expressed compared to day 3 hepatocytes, and CK19, AFP, EPCAM, and SOX9 proteins were highly expressed. Nuclei were stained with 4′,6-diamidino-2-phenylindole. Scale bar: 100 μm.

Article Snippet: The primary antibodies used were goat anti-dog albumin (A40-113A; Bethyl Laboratories Inc., Montgomery, TX, USA), mouse anti-human E-cadherin (sc-8426; Santa Cruz Biotechnology, Dallas, TX, USA), mouse anti-human MRP2 (MON9027; Cell Sciences, Newbury Port, MA, USA), rabbit anti-human EPCAM (21050-1-AP; Proteintech Rosemont, IL, USA), and rabbit anti-human SOX9 (LS-B12075; LS Bio (Absolute Biotech), Boston, MA, USA), rabbit anti-human Ck19 (10712-1-AP; Proteintech), and goat anti-human AFP (sc-8108; Santa Cruz Biotechnology).

Techniques: Staining, Immunostaining, Cell Differentiation

Journal: Regenerative Therapy

Article Title: Reprogramming canine cryopreserved hepatocytes to hepatic progenitor cells using small molecule compounds

doi: 10.1016/j.reth.2024.09.003

Figure Lengend Snippet: Antigen-positive cells before and after inducing differentiation. The graph shows the percentage of cells stained with ALB, MRP2, AFP, EpCAM, SOX9, and CK19 in mature hepatocytes (day 3) and hepatic progenitor cells (day 21) (400–800 cells were counted for each cell). The antigen-positive cells on day 3 and day 21 were compared using a t -test and a statistically significant difference was considered when ∗p < 0.05.

Article Snippet: The primary antibodies used were goat anti-dog albumin (A40-113A; Bethyl Laboratories Inc., Montgomery, TX, USA), mouse anti-human E-cadherin (sc-8426; Santa Cruz Biotechnology, Dallas, TX, USA), mouse anti-human MRP2 (MON9027; Cell Sciences, Newbury Port, MA, USA), rabbit anti-human EPCAM (21050-1-AP; Proteintech Rosemont, IL, USA), and rabbit anti-human SOX9 (LS-B12075; LS Bio (Absolute Biotech), Boston, MA, USA), rabbit anti-human Ck19 (10712-1-AP; Proteintech), and goat anti-human AFP (sc-8108; Santa Cruz Biotechnology).

Techniques: Staining

Journal: Scientific Reports

Article Title: Improved functionality of hepatic spheroids cultured in acoustic levitation compared to existing 2D and 3D models

doi: 10.1038/s41598-024-72059-x

Figure Lengend Snippet: Primary and secondary antibodies used for immunofluorescence.

Article Snippet: Mouse anti-MRP2 , Santa Cruz , SC-59609.

Techniques: Immunofluorescence

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Quantifying the transport of biologics across intestinal barrier models in real-time by fluorescent imaging

doi: 10.3389/fbioe.2022.965200

Figure Lengend Snippet: Verification of a differentiated and polarized epithelial monolayer using direct in-chip immunostaining. (A) Tight junction formation depicted by maximum intensity projection (MIP) from confocal z-stack of ZO-1 (green) and DAPI (blue) (scale bar is 100 µm). (B) Bottom, brush borders are visualized as MIP from confocal z-stack of ezrin (red) and DAPI (blue) (scale bar is 100 µm). Top, the apical orientation of ezrin expression is depicted as a vertical cross-section of the cell-tubule (Scale bar is 10 µm). (C) Bottom, BCRP efflux transporter (white) and DAPI (blue) are visualized as MIP from confocal z-stack (scale bar is 100 µm). Top, A vertical cross section of the BCRP stained cell-tubule. (D) Bottom, MIP of cell-tubule stained for the efflux transporter MRP2 (yellow) (scale bar is 100 µm). Top, vertical cross section of the MRP2 stained cell-tubule. (E) Brightfield microscopy images showing Alcian Blue staining of acidic mucins in mono- (top) and coculture (bottom) tubules (scale bar is 200 µm). (F) Micrograph of single z-slice of the cell layer along the ECM interface for mono- and coculture depicting Muc2 (green) and DAPI (blue) (scale bar is 100 µm).

Article Snippet: Primary antibodies against Rabbit-a-Zonula occludens-1 (ZO-1) (61–7,300, Thermo Fischer, 1:200), Rabbit-a-Mucin 2 (Muc2) (PA5-21329, Thermo Fischer, 1:200), Mouse-a-Ezrin (610,602, BD Transduction, 1:100), Mouse-a-Multidrug Resistance Protein 2 (MRP2) (SC-59608, Santa Cruz, 100 μg ml-1), Mouse-a-Breast Cancer Resistance Protein (BCRP) (ab3380, Abcam, 1:50) were used.

Techniques: Immunostaining, Expressing, Staining, Microscopy